Description
PNC-27 Peptide
PNC-27 is a synthetic peptide derived from a fragment of the p53 tumor suppressor protein and engineered to include a membrane-penetrating domain. The peptide sequence combines a region that targets the HDM-2 (MDM2) protein with a transmembrane-penetrating component, which researchers utilize in laboratory and preclinical settings to study protein-membrane interactions and cellular mechanisms related to selective membrane activity.
PNC-27 has been investigated in research focused on how engineered peptides interact with cell membranes, particularly in models examining tumor cell membranes expressing HDM-2, where the peptide may bind to membrane-associated HDM-2 and engage with membrane structure dynamics. This interaction has been associated with formation of membrane pores and selective disruption of membrane integrity in controlled research models.
Research on PNC-27 often examines its proposed ability to target cells with high levels of HDM-2 expression and its effects on membrane-associated processes in laboratory settings. Such studies involve measuring outcomes like membrane pore formation, lactate dehydrogenase (LDH) release as a marker of membrane disruption, and differential effects in transformed versus untransformed cell lines.
Chemical Makeup
Molecular Formula: C₁₈₈H₂₉₃N₅₃O₄₄S
Molecular Weight: 4031.7 g/mol
Sequence: Pro-Pro-Leu-Ser-Gln-Glu-Thr-Phe-Ser-Asp-Leu-Trp-Lys-Leu-Leu-Lys-Lys-Trp-Lys-Met-Arg-Arg-Asn-Gln-Phe-Trp-Val-Lys-Val-Gln-Arg-Gly-OH
Research and Experimental Context
PNC-27 and Membrane-Targeted Interactions
In preclinical studies, PNC-27 is used to investigate how peptide sequences with HDM-2 affinity interact with membranes exhibiting elevated HDM-2 expression, as seen in certain cancer cell lines. Experimental results suggest that the peptide may colocalize with membrane-bound HDM-2 and participate in pore-forming events that compromise membrane integrity.
Selectivity in Research Models
Some research indicates differential interactions between PNC-27 and transformed versus untransformed cells in vitro, leading to hypotheses about selective membrane engagement based on HDM-2 expression profiles.
PNC-27 peptide is available for research and laboratory purposes only. Please review and adhere to the site’s Terms and Conditions before ordering.
